ConductOrganoid

About

A combinatorial in vitro/in silico NAM for automated neurodevelopmental phenotyping of prenatal opioid exposure.

ConductOrganoid

ConductOrganoid is a combinatorial New Approach Methodology (NAM) that integrates two complementary modalities: in vitro human iPSC-derived neural organoid culture and in silico automated computational image analysis. The platform addresses the growing crisis of prenatal opioid exposure, where approximately 80,000 infants per year are born in the United States with Neonatal Opioid Withdrawal Syndrome (NOWS).

The ConductImage module extracts 16 morphological, intensity, and texture features from standard brightfield microscopy images and applies rigorous non-parametric statistics to detect neurodevelopmental phenotypes — entirely automated, observer-independent, and animal-free. Published studies show that opioid compounds (methadone, buprenorphine, fentanyl) cause the same types of morphological changes our pipeline is validated to detect.

Our Approach

A three-phase plan progressing from feasibility demonstration to regulatory-grade validation.

1

Phase 1 — Feasibility

Validate that the ConductImage pipeline reliably detects neurodevelopmental morphological differences using published organoid data (1,407 images, 64 organoids, 4 cell lines, 2 labs). Establish that validated features map to published opioid-organoid effects.

Current phase

2

Phase 2 — Validation

Apply the validated pipeline to opioid-exposed organoids (methadone, buprenorphine, fentanyl at 3 concentrations each). Establish dose-response morphological relationships. 3+ lab reproducibility study with FAIR-compliant NDHCC data submission. Integrate electrophysiology (ConductSignal).

3

Phase 3 — Translation

Working platform transfer to VQN with operating instructions. Comprehensive opioid dose-response dataset. Documentation for independent testing, validation, and regulatory qualification through the Complement-ARIE VQN framework.

The Team

The ConductOrganoid team combines academic and industry expertise across clinical medicine, addiction biology, computational engineering, and statistics.

Shuhan He, MD

PI / Clinical Translation

MGH / Harvard Medical School; CEO, ConductScience

Platform architecture, statistical framework, project leadership.

Shivani Pimparkar, MS

Co-PI / Addiction Biology & Disease Modeling

Boston University Bioinformatics; MGH Translational Research

Biological validation, opioid-organoid evidence base, Phase 2 experimental design.

Yijian Henry He, PhD

Technical Lead

PhD Economics; CTO, ConductScience

System architecture, cloud infrastructure, API development.

Louise Corscadden, PhD

Community & Governance

PhD Molecular Genetics; Director, ConductScience

Regulatory coordination, FAIR compliance, partnerships.

Bifei Hao, MS

Statistical Validation

Northwestern University Electrical Engineering

Statistical integrity, validation methodology.

Boyu Peng, MS

Platform Engineering

ConductScience

Deployment pipeline, reproducibility verification.

Pedram Safari, PhD

Mathematical Foundations

MGH Institute of Health Professions

Statistical framework, formal properties.

Santosh Adhikari, MS

ML & Interpretability

Computer vision and robotics; ConductScience

Pipeline interpretability, extension modules.

ConductScience, Inc.

Scientific equipment, software, and data services for the research community. ConductScience provides the infrastructure for developing, deploying, and distributing computational tools for biomedical research.

conductscience.com

Contact

For questions about the ConductOrganoid platform, the analysis pipeline, or collaboration opportunities:

Explore the Platform

See the analysis results, compare disease models, or read about our computational methods.